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Image Search Results
Journal: Journal of Hepatocellular Carcinoma
Article Title: Phellodendronoside A Exerts Anticancer Effects Depending on Inducing Apoptosis Through ROS/Nrf2/Notch Pathway and Modulating Metabolite Profiles in Hepatocellular Carcinoma
doi: 10.2147/JHC.S403630
Figure Lengend Snippet: PDA inhibited cell proliferation via the ROS/Nrf2/Notch signaling pathway in SMMC-7721 cells. ( A ) DCFH-DA staining presented the level of ROS after 24 h of treatment. ( B ) Fluo-4 (AM) staining presented the level of Ca 2+ after 24 h of treatment. ( C ) After 24 h of exposure to PDA, the expressions of Notch1, Jagged, Hes1, MCUR1, Nrf2 and HO-1 were analyzed by Western blotting. ( D – I ) The quantification results of Notch1, Jagged, Hes1, MCUR1, Nrf2 and HO-1 were analyzed by grayscale of blots. The data of three independent experiments were demonstrated as mean ± SD. * p < 0.05 and ** p < 0.01 indicating statistical differences compared with the control group.
Article Snippet: Ultra-High-Performance Liquid Chromatography (Dionex, USA); High-speed refrigerated centrifuge (Eppendorf, Germany); GAPDH (GB15002), Caspase3 (GB11009-1), Hes1 (GB11374), CyclinD1 (GB111935), CyclinE1 (GB111938), BCL-2 (GB113375), Jagged (GB11122), Notch1 (GB111690), horseradish peroxidase (HRP) conjugated goat anti-mouse (GB25301) and goat anti-rabbit (GB23303), and Bax (GB12690) were purchased from Wuhan servicebio Technology CO., LTD.
Techniques: Staining, Western Blot, Control